Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens

Jan W M van der Linden; Eveline Snelders; Jan P Arends; Simon M Daenen; Willem J G Melchers; Paul E Verweij

doi:10.1128/JCM.02221-09

Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens

J Clin Microbiol. 2010 Apr;48(4):1478-80. doi: 10.1128/JCM.02221-09. Epub 2010 Jan 27.

Authors

Jan W M van der Linden¹, Eveline Snelders, Jan P Arends, Simon M Daenen, Willem J G Melchers, Paul E Verweij

Affiliation

¹ Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands.

Abstract

We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.

Publication types

Case Reports

MeSH terms

Antifungal Agents / pharmacology*
Aspergillosis / diagnosis*
Aspergillosis / microbiology
Aspergillus / drug effects*
Aspergillus / genetics
Aspergillus / isolation & purification
Azoles / pharmacology*
Clinical Laboratory Techniques / methods*
DNA Primers / genetics
DNA, Fungal / genetics
Drug Resistance, Fungal*
Humans
Male
Middle Aged
Polymerase Chain Reaction / methods*

Substances

Antifungal Agents
Azoles
DNA Primers
DNA, Fungal