Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella

J Med Microbiol. 1991 Mar;34(3):175-80. doi: 10.1099/00222615-34-3-175.

Abstract

Twenty-six monoclonal antibodies (MAbs) directed against seven brucella outer-membrane proteins (OMPs) of 10, 16.5, 19, 25-27, 31-34, 36-38 and 89 Kda were screened for passive protection of mice; three MAbs (directed against 16.5, 25-27 and 36-38 Kda) reduced significantly the initial colonisation of the spleen measured 7 days after challenge. Although significant, the reduction in numbers of Brucella organisms in the spleen was low compared with that conferred by MAbs against lipopolysaccharide of smooth specificity (S-LPS). The three most protective MAbs belonged to two isotypes (IgG1 and IgG2a) and were specific for three different OMPs. No relationship between protection and binding of MAbs to the challenge S strain or its rough mutant was observed in the mouse model. The humoral protection depended mainly on antibodies directed against S-LPS, although some MAbs against OMPs had weak protective activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Bacterial / immunology*
  • Antigens, Surface / immunology
  • Bacterial Outer Membrane Proteins / immunology*
  • Blotting, Western
  • Brucella / immunology*
  • Cell Membrane / immunology
  • Mice
  • Mice, Inbred BALB C
  • Models, Biological

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Antigens, Surface
  • Bacterial Outer Membrane Proteins