The aim of the study was to assess the normal and synostosed coronal cranial sutures morphology at an ultrastructural level. Different operative specimens of human coronal sutures were collected. Three-dimensional reconstruction of the microcomputed tomography (micro-CT) slices were obtained and compared with the representative histologic sections. The micro-CT scanning provides a three-dimensional view of the sutures at a microscopical level thus allowing to establish the stage of development and the bone morphology. The micro-CT has the advantage of being able to image more slices than the normal histology without any damage to the analyzed specimens. Micro-CT is a powerful tool in the craniofacial area that allows us to obtain many structure-orientated slices and in conjunction with the histologic sections provides an overall thorough quantitative analysis of all cranial sutures.