Abstract
Tertiary sulfonamides were identified in a HTS as dual liver X receptor (LXR, NR1H2, and NR1H3) ligands, and the binding affinity of the series was increased through iterative analogue synthesis. A ligand-bound cocrystal structure was determined which elucidated key interactions for high binding affinity. Further characterization of the tertiary sulfonamide series led to the identification of high affinity LXR antagonists. GSK2033 (17) is the first potent cell-active LXR antagonist described to date. 17 may be a useful chemical probe to explore the cell biology of this orphan nuclear receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Crystallography, X-Ray
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Haplorhini
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Humans
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Liver X Receptors
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Models, Molecular
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Orphan Nuclear Receptors / antagonists & inhibitors*
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Orphan Nuclear Receptors / genetics
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Structure-Activity Relationship
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Sulfonamides / chemical synthesis*
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Sulfonamides / chemistry
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Sulfonamides / pharmacology
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Transcriptional Activation / drug effects
Substances
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2,4,6-trimethyl-N-((3'-(methylsulfonyl)-4-biphenylyl)methyl)-N-((5-(trifluoromethyl)-2-furanyl)methyl)benzenesulfonamide
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Liver X Receptors
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NR1H2 protein, human
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NR1H3 protein, human
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Orphan Nuclear Receptors
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Sulfonamides