Abstract
We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Brain Abscess / microbiology*
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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DNA, Ribosomal / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Fusobacterium nucleatum / classification
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Fusobacterium nucleatum / genetics
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Fusobacterium nucleatum / isolation & purification*
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Gram-Negative Bacterial Infections / diagnosis*
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Gram-Negative Bacterial Infections / microbiology
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Humans
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Male
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction
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Porphyromonas endodontalis / classification
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Porphyromonas endodontalis / genetics
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Porphyromonas endodontalis / isolation & purification*
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S