SCID mice model in vivo evaluation of autologous and allogeneic dendritic cells activity on B-cell chronic lymphocytic leukemia

Folia Histochem Cytobiol. 2009;47(4):563-70. doi: 10.2478/v10042-008-0101-9.

Abstract

In the present study we investigated in vivo therapeutic potential of DCs vaccines in B-cell chronic lymphocytic leukemia (B-CLL). On the day 0 the SCID mice were intraperitoneally inoculated with peripheral blood mononuclear cells (PBMC) of B-CLL patients at a dose of 10-30 x 10(6) and left untreated (controls) or i.p. injected on the day 7 with 0.2 - 14.0 x 10(6) dendritic cells. DCs were generated in vitro from peripheral blood monocytes of B-CLL donors (autologous DCs) or healthy donors (allogeneic cells) and pulsed with B-CLL antigens. On the day 14, the effect of implanted cells interactions was evaluated by a counting of CD19+CD5+ human leukemic cells and human T cells in the peritoneal fluid of mice. We found, that mean numbers of CD19+CD5+ leukemic cells as well as human T cells were lowered in peritoneal fluid of mice treated with allogeneic APCs. However, we did not observe similar effects with autologous DCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Antigens, CD19 / immunology
  • CD5 Antigens / immunology
  • Cancer Vaccines / immunology*
  • Dendritic Cells* / immunology
  • Dendritic Cells* / transplantation
  • Disease Models, Animal
  • Female
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell* / drug therapy
  • Leukemia, Lymphocytic, Chronic, B-Cell* / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, SCID*
  • Middle Aged
  • T-Lymphocytes / immunology
  • Transplantation, Autologous*
  • Transplantation, Homologous*

Substances

  • Antigens, CD19
  • CD5 Antigens
  • Cancer Vaccines