On the identification of a conjugated diene component of duodenal bile as 9Z,11E-octadecadienoic acid

G N Smith; M Taj; J M Braganza

doi:10.1016/0891-5849(91)90016-v

On the identification of a conjugated diene component of duodenal bile as 9Z,11E-octadecadienoic acid

Free Radic Biol Med. 1991;10(1):13-21. doi: 10.1016/0891-5849(91)90016-v.

Authors

G N Smith¹, M Taj, J M Braganza

Affiliation

¹ Department of Chemistry, University of Manchester, England.

PMID: 2050295
DOI: 10.1016/0891-5849(91)90016-v

Abstract

The lipid free radical marker, termed diene conjugation, in secretin-stimulated human bile obtained from the duodenum, was shown by high performance liquid chromatography and capillary gas chromatography-mass spectrometry, to be due mainly to 9Z,11E-octadecadienoic acid. The lack of evidence for possible conjugated diene isomers argues for an enzymatic origin of this product rather than being due to a random free radical mechanism, as is usually assumed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bile Acids and Salts / chemistry
Bile Acids and Salts / metabolism*
Biomarkers
Chromatography, High Pressure Liquid
Duodenum / drug effects
Duodenum / metabolism*
Free Radicals
Humans
Linoleic Acids / metabolism*
Linoleic Acids, Conjugated*
Secretin / pharmacology

Substances

Bile Acids and Salts
Biomarkers
Free Radicals
Linoleic Acids
Linoleic Acids, Conjugated
Secretin
9,11-linoleic acid