A novel water-soluble polymer, poly[(chloromethoxytrialanine methyl ester)phosphazene] (poly-Tame), was characterized and evaluated using high-performance size-exclusion chromatography, gradient reversed-phase high-performance liquid chromatography and ion chromatography. These novel liquid chromatographic methods were validated for application to in vitro biodegradation experiments of poly-Tame in aqueous solutions. Results from method validation experiments are presented.