Physicochemical and biological properties of nano-hydroxyapatite-reinforced aliphatic polyurethanes membranes

J Biomater Sci Polym Ed. 2010;21(12):1619-36. doi: 10.1163/092050609X12524778957011. Epub 2010 Jun 9.

Abstract

Polymer nano-composite membranes, based on aliphatic biodegradable polyurethane (PU) elastomers and nano-hydroxyapatite (n-HA), were prepared by solvent casting and freeze-drying. The PU matrix was synthesized from 4,4'-dicyclohexylmethane diisocyanate (H(12) MDI), poly(ethylene glycol) (PEG), castor oil (CO) and 1,4-butandiol (BDO). The n-HA/PU membranes were characterized by SEM, XRD, IR, TG, mechanical test and in vitro biocompatibility. The results revealed that incorporation of 30 wt% n-HA into the PU matrix increased the tensile strength nearly by 186% and the elongation-at-break by 107% compared to pure PU. The addition of n-HA had the slight positive effect on the thermal stability of PU. Cell culture and MTT assays showed that the incorporation of n-HA into the PU matrix provided a favourable environment for initial cell adhesion, maintained cell viability and cell proliferation. These results suggested that the n-HA/PU composite membrane might be a prospective biodegradable guided bone regeneration (GBR) membrane for future applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Regeneration
  • Cell Adhesion / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chemical Phenomena*
  • Durapatite / chemistry*
  • Durapatite / pharmacology*
  • Humans
  • Materials Testing
  • Mechanical Phenomena
  • Membranes, Artificial*
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Nanostructures / chemistry*
  • Polyurethanes / chemistry*
  • Spectroscopy, Fourier Transform Infrared
  • Thermogravimetry

Substances

  • Membranes, Artificial
  • Polyurethanes
  • Durapatite