ERIC-PCR genotyping of Haemophilus parasuis isolates from Brazilian pigs

Nubia Resende Macedo; Simone Rodrigues Oliveira; Andrey Pereira Lage; José Lúcio Santos; Marina Rios Araújo; Roberto Maurício Carvalho Guedes

doi:10.1016/j.tvjl.2010.05.024

ERIC-PCR genotyping of Haemophilus parasuis isolates from Brazilian pigs

Vet J. 2011 Jun;188(3):362-4. doi: 10.1016/j.tvjl.2010.05.024. Epub 2010 Jun 26.

Authors

Nubia Resende Macedo¹, Simone Rodrigues Oliveira, Andrey Pereira Lage, José Lúcio Santos, Marina Rios Araújo, Roberto Maurício Carvalho Guedes

Affiliation

¹ Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, P.O. Box 567, Belo Horizonte, Brazil.

PMID: 20580292
DOI: 10.1016/j.tvjl.2010.05.024

Abstract

Among 63 Haemophilus parasuis isolates from 17 Brazilian pig herds, 33 genotypes were identified by enterobacterial repetitive intergenic consensus (ERIC)-PCR, with a diversity index of 0.96. Eight serovars were detected, with serovar 4 (15.9%) being most frequent; 60.3% of isolates were non-typeable. There was no strong association between site of isolation and genotype or serovar.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brazil
Genotype
Haemophilus Infections / microbiology
Haemophilus Infections / veterinary*
Haemophilus parasuis / classification
Haemophilus parasuis / genetics*
Haemophilus parasuis / isolation & purification
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / veterinary
Swine
Swine Diseases / microbiology*