Abstract
Accumulation of plasma advanced oxidation protein products (AOPPs) promotes progression of proteinuria and glomerulosclerosis. To investigate the molecular basis of AOPPs-induced proteinuria, normal Sprague-Dawley rats were treated with AOPPs-modified rat serum albumin. The expression of glomerular podocyte slit diaphragm (PSD)-associated proteins, nephrin and podocin, was significantly decreased coincident with the onset of albuminuria in rats treated with AOPPs. Chronic inhibition of NADPH oxidase by apocynin prevented down-regulation of nephrin and podocin and decreased albuminuria in AOPPs-challenged rats. This suggested that accumulation of AOPPs promotes proteinuria, possibly via down-regulating the expression of PSD-associated proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Cells, Cultured
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Dose-Response Relationship, Drug
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Gene Expression / drug effects
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Kidney Glomerulus / drug effects
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Kidney Glomerulus / metabolism
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Male
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Oxidation-Reduction
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Podocytes / cytology
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Podocytes / drug effects*
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Podocytes / metabolism
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Proteins / chemistry
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Proteinuria / chemically induced
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Proteinuria / metabolism
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Rats
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Rats, Sprague-Dawley
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Reactive Oxygen Species / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Serum Albumin / chemistry
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Serum Albumin / pharmacology*
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p38 Mitogen-Activated Protein Kinases / metabolism*
Substances
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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NPHS2 protein
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Proteins
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Reactive Oxygen Species
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Serum Albumin
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nephrin
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p38 Mitogen-Activated Protein Kinases