Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis

J Clin Microbiol. 2010 Sep;48(9):3410-3. doi: 10.1128/JCM.00718-10. Epub 2010 Jul 14.

Abstract

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteriological Techniques / methods*
  • Blood / microbiology*
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Time Factors
  • Transition Temperature

Substances

  • DNA, Bacterial