Characterization and expression of a sea star otx ortholog (Protxβ1/2) in the larva of Patiriella regularis

Gene Expr Patterns. 2010 Oct-Dec;10(7-8):323-7. doi: 10.1016/j.gep.2010.07.003. Epub 2010 Jul 18.

Abstract

A transcript of otx from the sea star Patiriella regularis (Protxβ1/2) was characterized and its expression in early bipinnaria larvae was documented by whole mount in situ hybridization (WMISH). The nucleotide sequence exhibited 94% identity with Amotxβ1/2 from the closely related species Patiria miniata. Protxβ1/2 was expressed strongly in the developing archenteron in the future fore and mid-gut regions. This was followed by expression of Protxβ1/2 in the developing enterocoels, mesodermal derivatives. This suggests a role for Protx in endomesoderm development. In coelom development, Protxβ1/2 was first expressed in the left coelom. Subsequently expression was evident in the right coelom, but localization was never as strong as in the left coelom. This asymmetry in Protxβ1/2 expression in the coeloms was evident up to the stage when they started to extend posteriorly. These data indicate that Protxβ1/2 may have a role in coelom development, particularly in the left coelom, a definitive adult structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gastrula / embryology*
  • Gastrula / metabolism
  • Gene Expression
  • In Situ Hybridization
  • Larva / genetics
  • Larva / metabolism
  • Mesoderm / embryology*
  • Mesoderm / metabolism
  • Molecular Sequence Data
  • Otx Transcription Factors / chemistry
  • Otx Transcription Factors / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Starfish / embryology*
  • Starfish / genetics*
  • Starfish / metabolism

Substances

  • Otx Transcription Factors