Immunohistochemical expression of CD3, CD20, CD45, CD68 and bcl-2 in oral squamous cell carcinoma

Anal Quant Cytol Histol. 2010 Apr;32(2):70-7.

Abstract

Objective: To study the main processes involved in oral squamous cell carcinoma (OSCC): apoptosis and inflammation.

Study design: The immunohistochemical expression of bcl-2, CD3, CD20, CD45 and CD68 were studied by standard linked streptavidin-biotin horseradish peroxidase technique in 21 paraffin-embedded OSCC specimens in order to establish their possible correlation with the degree of tumoral differentiation.

Results: A trend was observed for the association of inflammatory infiltrate with the degree of tumor differentiation: well and moderately differentiated tumors tend to be associated to a dense inflammatory infiltrate (57.9% of cases), while poorly differentiated cancers seem to be associated to a low inflammatory infiltrate in 85.7% of cases. The most expressed molecule was CD68, followed by CD45, CD20 and CD3. Bcl-2 was low or moderately expressed, and immunostaining was more diffuse in moderately or poorly differentiated oral cancers.

Conclusion: Inflammatory infiltrate and the degree of OSCC differentiation may be linked, but the specific role still remains unclear.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD / metabolism*
  • Apoptosis
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cell Count
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Immunoenzyme Techniques
  • Lymphocytes, Tumor-Infiltrating / metabolism
  • Lymphocytes, Tumor-Infiltrating / pathology
  • Male
  • Middle Aged
  • Mouth Neoplasms / metabolism*
  • Mouth Neoplasms / pathology
  • Neoplasm Staging
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Young Adult

Substances

  • Antigens, CD
  • Biomarkers, Tumor
  • Proto-Oncogene Proteins c-bcl-2