Structure of the O-polysaccharide of Acinetobacter sp. VS-15 and Acinetobacter lwoffii EK67

Nikolay P Arbatsky; Anna N Kondakova; Alexander S Shashkov; Marina S Drutskaya; Pavel V Belousov; Sergei A Nedospasov; Mayya A Petrova; Yuriy A Knirel

doi:10.1016/j.carres.2010.07.031

Structure of the O-polysaccharide of Acinetobacter sp. VS-15 and Acinetobacter lwoffii EK67

Carbohydr Res. 2010 Oct 13;345(15):2287-90. doi: 10.1016/j.carres.2010.07.031. Epub 2010 Jul 27.

Authors

Nikolay P Arbatsky¹, Anna N Kondakova, Alexander S Shashkov, Marina S Drutskaya, Pavel V Belousov, Sergei A Nedospasov, Mayya A Petrova, Yuriy A Knirel

Affiliation

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.

PMID: 20732679
DOI: 10.1016/j.carres.2010.07.031

Abstract

An O-polysaccharide was released by mild acid degradation of the lipopolysaccharide of Acinetobacter sp. VS-15 and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC and HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: [Formula: see text]. The O-polysaccharide of Acinetobacter lwoffii EK67 was found to have the same structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter / chemistry*
Carbohydrate Sequence
Lipopolysaccharides / chemistry*
Lipopolysaccharides / isolation & purification
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Polysaccharides, Bacterial / chemistry*
Polysaccharides, Bacterial / isolation & purification

Substances

Lipopolysaccharides
Polysaccharides, Bacterial