The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction

J Immunol. 2010 Oct 1;185(7):4457-69. doi: 10.4049/jimmunol.1001782. Epub 2010 Aug 30.

Abstract

Systemic lupus erythematosus (SLE) is characterized by increased vascular risk due to premature atherosclerosis independent of traditional risk factors. We previously proposed that IFN-α plays a crucial role in premature vascular damage in SLE. IFN-α alters the balance between endothelial cell apoptosis and vascular repair mediated by endothelial progenitor cells (EPCs) and myeloid circulating angiogenic cells (CACs). In this study, we demonstrate that IFN-α promotes an antiangiogenic signature in SLE and control EPCs/CACs, characterized by transcriptional repression of IL-1α and β, IL-1R1, and vascular endothelial growth factor A, and upregulation of IL-1R antagonist and the decoy receptor IL-1R2. IL-1β promotes significant improvement in the functional capacity of lupus EPCs/CACs, therefore abrogating the deleterious effects of IFN-α. The beneficial effects from IL-1 are mediated, at least in part, by increases in EPC/CAC proliferation, by decreases in EPC/CAC apoptosis, and by preventing the skewing of CACs toward nonangiogenic pathways. IFN-α induces STAT2 and 6 phosphorylation in EPCs/CACs, and JAK inhibition abrogates the transcriptional antiangiogenic changes induced by IFN-α in these cells. Immunohistochemistry of renal biopsies from patients with lupus nephritis, but not anti-neutrophil cytoplasmic Ab-positive vasculitis, showed this pathway to be operational in vivo, with increased IL-1R antagonist, downregulation of vascular endothelial growth factor A, and glomerular and blood vessel decreased capillary density, compared with controls. Our study introduces a novel putative pathway by which type I IFNs may interfere with vascular repair in SLE through repression of IL-1-dependent pathways. This could promote atherosclerosis and loss of renal function in this disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Atherosclerosis / immunology
  • Atherosclerosis / metabolism*
  • Cell Differentiation / physiology
  • Endothelial Cells / metabolism
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Humans
  • Immunohistochemistry
  • Interferon-alpha / metabolism*
  • Interleukin 1 Receptor Antagonist Protein / biosynthesis
  • Interleukin-1 / immunology
  • Interleukin-1 / metabolism*
  • Kidney / blood supply
  • Kidney / metabolism
  • Kidney / pathology
  • Lupus Erythematosus, Systemic / genetics
  • Lupus Erythematosus, Systemic / metabolism*
  • Lupus Erythematosus, Systemic / pathology
  • Lupus Nephritis / metabolism
  • Lupus Nephritis / pathology
  • Male
  • Microscopy, Fluorescence
  • Neovascularization, Physiologic*
  • Oligonucleotide Array Sequence Analysis
  • Receptors, Interleukin-1 / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / physiology
  • Stem Cells
  • Vascular Endothelial Growth Factor A / biosynthesis

Substances

  • Interferon-alpha
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Receptors, Interleukin-1
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A