Maternal smoking and impaired endothelium-dependent nitric oxide-mediated relaxation of uterine small arteries in vitro

Am J Obstet Gynecol. 2011 Feb;204(2):177.e1-7. doi: 10.1016/j.ajog.2010.09.006. Epub 2010 Oct 25.

Abstract

Objective: This study aimed to investigate the endothelium-dependent relaxation of uterine small arteries from pregnant nonsmokers, smokers, and ex-smokers who stopped smoking early in pregnancy.

Study design: Uterine arteries were dissected from myometrial biopsies obtained during elective cesarean sections of 34 uncomplicated, singleton pregnancies, and the vascular function was assessed in a wire myograph for isometric recordings. Serum cotinine verified self-reported smoking; 15 were nonsmokers, 10 were smokers, and 9 were ex-smokers.

Results: Arteries from smokers and ex-smokers had reduced bradykinin-induced relaxation compared to arteries from nonsmokers (P < .05). The relaxation response to the nitric oxide donor sodium nitroprusside was similar in arteries from nonsmokers and smokers but was better in arteries from ex-smokers (P < .05).

Conclusion: The findings suggest that maternal smoking reduces endothelium-dependent nitric oxide-mediated relaxation in uterine small arteries and that smoking cessation early in pregnancy does not fully abolish this deleterious effect, despite improvement in relaxation to nitroprusside.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Area Under Curve
  • Arteries / metabolism
  • Arteries / physiopathology*
  • Chi-Square Distribution
  • Cotinine / blood
  • Endothelium, Vascular / metabolism
  • Endothelium, Vascular / physiopathology*
  • Female
  • Humans
  • Myography
  • Nitric Oxide / metabolism*
  • Pregnancy
  • Smoking*
  • Surveys and Questionnaires
  • Uterus / blood supply*
  • Uterus / metabolism
  • Uterus / physiopathology
  • Vasodilation / physiology*

Substances

  • Nitric Oxide
  • Cotinine