Cynomolgus macaque CYP4 isoforms are functional, metabolizing arachidonic acid

J Vet Med Sci. 2011 Apr;73(4):487-90. doi: 10.1292/jvms.10-0333. Epub 2010 Nov 19.

Abstract

Cytochrome P450 (CYP) is important for metabolism of not only xenobiotics such as drugs, but also endogenous compounds including arachidonic acids. CYP4A11, CYP4F3v2, CYP4F11, and CYP4F45 have been identified in cynomolgus macaque, an animal species widely used for investigation of drug metabolism due to its evolutionary closeness to human. However, their metabolic functions have not been investigated. In this study, proteins were heterologously expressed in Escherichia coli and characterized by metabolic assays using arachidonic acids as substrates that are metabolized by CYP4 isoforms in human. The results showed that all four CYPs metabolized arachidonic acids. Therefore, cynomolgus macaque CYP4A11, CYP4F3v2, CYP4F11, and CYP4F45 are functional enzymes.

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism*
  • Cytochrome P-450 CYP4A / classification*
  • Cytochrome P-450 CYP4A / genetics
  • Cytochrome P-450 CYP4A / metabolism
  • Humans
  • Isoenzymes
  • Macaca fascicularis / metabolism*
  • Phylogeny
  • Substrate Specificity

Substances

  • Isoenzymes
  • Arachidonic Acid
  • Cytochrome P-450 CYP4A