Abstract
Perfluorooctane sulfonate (PFOS) is an environmental persistent acid found at low levels in human, wildlife, and environmental media samples. To study the apoptosis effects of PFOS on microglia, murine N9 cell line was used as a model in current research. The results showed that PFOS could reduce the cell viability significantly, and the cellular apoptosis induced by PFOS was closely accompanied with dissipation of mitochondria membrane potential, upregulation messenger RNAs (mRNAs) of p53, Bax, caspase 9, and caspase 3, and decreased expression of Bcl-2 mRNA. These results suggested that PFOS could disturb homeostasis of N9 cells, impact mitochondria, and affect gene expression of apoptotic regulators, all of which resulted in a start-up of apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkanesulfonic Acids / toxicity*
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Animals
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Apoptosis / drug effects*
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Biomarkers
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Caspase 3 / genetics
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Caspase 3 / metabolism
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Caspase 9 / genetics
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Caspase 9 / metabolism
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Cell Line
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Cell Survival / drug effects
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Cytotoxins / toxicity
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Fluorocarbons / toxicity*
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Gene Expression Regulation
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Homeostasis
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Mice
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Microglia / cytology*
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Microglia / pathology
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Mitochondria / drug effects
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Mitochondria / metabolism
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Mitochondrial Membranes / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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bcl-2-Associated X Protein / genetics
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bcl-2-Associated X Protein / metabolism
Substances
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Alkanesulfonic Acids
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Bax protein, mouse
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Biomarkers
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Cytotoxins
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Fluorocarbons
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RNA, Messenger
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bcl-2-Associated X Protein
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perfluorooctane sulfonic acid
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Casp3 protein, mouse
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Casp9 protein, mouse
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Caspase 3
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Caspase 9