Supplemental ultraviolet-B and ozone: impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv. Padmini)

Abstract

The present investigation used Linum usitatissimum L. cv. Padmini (linseed), under field conditions in open-top chambers, to evaluate the interactive effects of supplemental ultraviolet-B (sUV-B; ambient +7.2 kJ · m(-2) · d(-1)) and ozone (O(3); ambient +10 ppb). Treatment of plants with sUV-B and O(3) , individually or in combination, caused several changes in enzymatic and non-enzymatic components of the antioxidant defence system. Photo-oxidative damage caused by sUV-B and O(3) , included lipid peroxidation, changed protein profiles and caused DNA strand breakage. One-dimensional gel electrophoresis revealed that proteins of 222.24 and 50.5 kDa are specific and appear after sUV-B and O(3) exposure, and could be used as indicator proteins. Effects of sUV-B and O(3) given separately are more detrimental as compared to combined treatment. Mutational and structural alterations in linseed DNA after these stresses were also examined using RAPD with ten different primers. The study concluded that both stresses, i.e. sUV-B and O(3) , are phytotoxic, causing significant changes in metabolites, antioxidants, the leaf proteome and the genome of linseed, but their interactive effect was always less than additive.