Thrombin expression in prostate: a novel finding

Cancer Invest. 2011 Jan;29(1):62-7. doi: 10.3109/07357907.2010.535057.

Abstract

Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue.

Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays.

Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05).

Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Epithelial Cells / chemistry
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Polymerase Chain Reaction
  • Prostate / chemistry*
  • Prostate / pathology
  • Prostatic Neoplasms / chemistry*
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / pathology
  • Prothrombin / analysis*
  • Prothrombin / genetics
  • RNA, Messenger / analysis
  • Stromal Cells / chemistry
  • Thrombin / analysis*
  • Thrombin / genetics
  • Tissue Array Analysis

Substances

  • RNA, Messenger
  • Prothrombin
  • Thrombin