Optimized intrapleural cisplatin chemotherapy with a fibrin carrier after extrapleural pneumonectomy: a preclinical study

J Thorac Cardiovasc Surg. 2011 Jan;141(1):65-71. doi: 10.1016/j.jtcvs.2010.09.032.

Abstract

Objective: Our objective was to evaluate whether platinum concentrations in chest wall tissue and in serum are optimized by intracavitary application of cisplatin loaded to a fibrin carrier compared with cisplatin solution in a randomized setting of a pig model.

Methods: After left-sided pneumonectomy including parietal pleurectomy, pigs were randomly assigned to receive either 90 mg/m(2) cisplatin intracavitary solution (n = 6) or to receive 5 mg cisplatin-fibrin (n = 5) applied on a predefined area of the chest wall. Platinum concentration in serum as well as in chest wall tissue was determined at several early time points until day 5 after treatment. Platinum levels were measured by inductively coupled plasma sector field mass spectrometric detection with a matrix-matched calibration procedure.

Results: The dose- and surface-corrected (geometric) mean concentration of cisplatin in chest wall tissue 2 hours but also at day 5 after the application was doubled in animals treated with cisplatin-fibrin compared with the animals treated with cisplatin-solution. In serum, the dose- and surface-corrected exposure toward cisplatin (area under the curve(0-5d)) was significantly lower with cisplatin-fibrin than with cisplatin-solution (P < .0005). This is also reflected by significantly reduced serum creatinine and urea values in the cisplatin-fibrin group (P < .0001). Animals treated with cisplatin-fibrin additionally had a significantly better postoperative course as assessed by a well-being score (P < .001).

Conclusions: After cisplatin-fibrin treatment, cisplatin tissue concentration was increased whereas systemic cisplatin concentrations were significantly reduced in comparison with cisplatin-solution treatment. This finding offers a clear advantage inasmuch as rate and severity of systemic adverse events can be reduced while local cytotoxic concentrations are at least maintained.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage*
  • Antineoplastic Agents / blood
  • Antineoplastic Agents / pharmacokinetics
  • Antineoplastic Agents / toxicity
  • Biomarkers / blood
  • Chemotherapy, Adjuvant
  • Cisplatin / administration & dosage*
  • Cisplatin / blood
  • Cisplatin / pharmacokinetics
  • Cisplatin / toxicity
  • Creatinine / blood
  • Drug Administration Routes
  • Drug Carriers*
  • Fibrin Tissue Adhesive*
  • Models, Animal
  • Pleural Cavity
  • Pneumonectomy / methods*
  • Spectrophotometry, Atomic
  • Sus scrofa
  • Thoracic Wall / metabolism
  • Tissue Distribution

Substances

  • Antineoplastic Agents
  • Biomarkers
  • Drug Carriers
  • Fibrin Tissue Adhesive
  • Creatinine
  • Cisplatin