Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment

J Synchrotron Radiat. 2011 Jan;18(1):88-91. doi: 10.1107/S0909049510037076. Epub 2010 Nov 5.

Abstract

Human hematopoietic prostaglandin synthase, one of the better therapeutic target enzymes for allergy and inflammation, was crystallized with 22 inhibitors and in three inhibitor-free conditions in microgravity. Most of the space-grown crystals showed better X-ray diffraction patterns than the terrestrially grown ones, indicating the advantage of a microgravity environment on protein crystallization, especially in the case of this protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization / methods*
  • Humans
  • Intramolecular Oxidoreductases / antagonists & inhibitors
  • Intramolecular Oxidoreductases / chemistry*
  • Lipocalins / antagonists & inhibitors
  • Lipocalins / chemistry*
  • Piperidines / chemistry
  • Space Flight
  • Weightlessness*
  • X-Ray Diffraction

Substances

  • 4-benzhydryloxy-1-(3-(1H-tetrazol-5-yl-)-propyl)piperidine
  • Lipocalins
  • Piperidines
  • Intramolecular Oxidoreductases
  • prostaglandin R2 D-isomerase