Aim: To study the alteration of nuclear envelope associated NTPase activity and mRNA nucleocytoplasmic transport in rabbit cardiomyocyte following in vitro ischemia and reperfusion.
Methods: The model of myocardial perfusion in rabbit was used to produce myocardial I/R injury and cardiomyocyte nuclear envelope vesicles were prepared by density gradient centrifugation for the assay of mRNA transport rate and NTPase activity.
Results: NTPase activity was reduced and mRNA transport rate was significantly decreased in I/R (P < 0.01) but not in ischemia group( P > 0.05).
Conclusion: The nuclear envelope vesicles had been injured following ischemia and reperfusion and resulted in NTPase activity reduced and egress of mRNA interrupted, and therefore may lead to decreasing of protein synthesis.