Cord blood plasma reference intervals for potential sepsis markers: pro-adrenomedullin, pro-endothelin, and pro-atrial natriuretic peptide

Clin Biochem. 2011 Mar;44(4):337-41. doi: 10.1016/j.clinbiochem.2010.12.012. Epub 2011 Jan 3.

Abstract

Objectives: To establish reference values in cord blood of the following new sepsis markers: pro-adrenomedullin (MR-proADM), pro-endothelin (CT-proET-1), and pro-atrial natriuretic peptide (MR-proANP).

Methods: MR-proADM, CT-proET-1, MR-proANP, and procalcitonin (PCT) were measured in cord blood of newborn infants by Time Resolved Amplified Cryptate Emission (TRACE) technology. The inclusion criteria in the control group (n=194) was the absence of any clinical sign or risk factor of sepsis. A group of 73 newborn infants presenting with risk factors of sepsis at delivery was also studied.

Results: The median values (reference interval) of CT-proET-1, MR-pro-ADM, and MR-proANP measured in cord blood plasma were 72 pmol/L (39-115), 0.84 nmol/L (0.5-1.38), and 163 pmol/L (76-389), respectively. The PCT reference interval was not significantly different from that previously described in cord blood serum.

Conclusions: The reference intervals established will serve as a starting point for further clinical investigations aimed to elucidate the potential prognostic/diagnostic value of these markers in neonatal sepsis management.

MeSH terms

  • Adrenomedullin / blood
  • Atrial Natriuretic Factor / blood
  • Biomarkers / blood*
  • Calcitonin / blood
  • Calcitonin Gene-Related Peptide
  • Endothelin-1 / blood
  • Female
  • Fetal Blood / chemistry*
  • Fetus
  • Humans
  • Infant
  • Infant, Newborn
  • Photoelectron Spectroscopy
  • Pregnancy
  • Pregnancy Complications, Infectious / blood*
  • Pregnancy Complications, Infectious / diagnosis
  • Pregnancy Complications, Infectious / physiopathology
  • Prognosis
  • Protein Precursors / blood
  • Reference Values
  • Sepsis / blood*
  • Sepsis / diagnosis
  • Sepsis / physiopathology

Substances

  • Biomarkers
  • CALCA protein, human
  • Endothelin-1
  • Protein Precursors
  • proendothelin 1
  • Adrenomedullin
  • Atrial Natriuretic Factor
  • Calcitonin
  • Calcitonin Gene-Related Peptide