Protein composition analysis of oil bodies from maize embryos during germination

J Plant Physiol. 2011 Mar 15;168(5):510-3. doi: 10.1016/j.jplph.2010.08.020. Epub 2011 Jan 17.

Abstract

Seed oil bodies (OBs) are intracellular particles that store lipids. In maize embryos, the oil bodies are accumulated mainly in the scutellum. Oil bodies were purified from the scutellum of germinating maize seeds and the associated proteins were extracted and subjected to 2-DE analysis followed by LC-MS/MS for protein identification. In addition to the previously known oil body proteins oleosin, caleosin and steroleosin, new proteins were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Chromatography, Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Germination*
  • Plant Proteins / metabolism*
  • Seeds / metabolism*
  • Tandem Mass Spectrometry
  • Zea mays / embryology*
  • Zea mays / physiology

Substances

  • Plant Proteins