Abstract
We identify a new mammalian cohesin subunit, RAD21-like protein (RAD21L), with sequence similarity to RAD21 and REC8. RAD21L localizes along axial elements in early meiotic prophase, in a manner that is spatiotemporally different to either REC8 or RAD21. Remarkably, RAD21L and REC8 have symmetrical, mutually exclusive localization on the not-yet-synapsed homologues, implying that the cohesin patterning could provide a code for homologue recognition. RAD21 transiently localizes to axial elements after the dissociation of RAD21L and REC8 in late pachytene, a period of recombination repair. Further, we show that the removal of cohesins and synaptonemal complex during late meiotic prophase is promoted by Polo-like kinase 1, which is similar to the mitotic prophase pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Centromere
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Chromatography, Liquid
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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Chromosome Pairing*
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Chromosome Segregation
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Chromosomes, Mammalian / metabolism*
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Cohesins
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DNA-Binding Proteins
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Mass Spectrometry
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Meiosis*
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Mice
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Molecular Sequence Data
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Nuclear Proteins / metabolism
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Phosphoproteins / metabolism
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Polo-Like Kinase 1
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Prophase
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Protein Serine-Threonine Kinases / metabolism
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Protein Subunits
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Proto-Oncogene Proteins / metabolism
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Synaptonemal Complex / metabolism
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Nuclear Proteins
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Phosphoproteins
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Protein Subunits
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Proto-Oncogene Proteins
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RAD21L protein, mouse
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Rad21 protein, mouse
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Rec8 protein, mouse
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Protein Serine-Threonine Kinases