In vivo graft performance of an improved bone substitute composed of poor crystalline hydroxyapatite based biphasic calcium phosphate

Dent Mater J. 2011;30(1):21-8. doi: 10.4012/dmj.2010-060. Epub 2011 Jan 26.

Abstract

The ability to promote new bone formation of poor crystalline hydroxyapatite (PC-HA) based biphasic calcium phosphate (BCP) bone substitutes were investigated. Various ratios of porous PC-HA/β-TCP (70/30, 60/40, and 0/100) grafts were fabricated. SEM and XRD measurements were performed to study the morphology and crystalline structure. Cylindrical artificial bone defects (3 × 6 mm(2)) were produced in alveolar bone at premolars extraction site and then filled with sterilized bone grafts. Commercial MBCP and unfilled empty defect served as control groups. At 8 weeks postoperation, samples were harvested from each artificial defect site for histological analysis. New bone formation of all the PC-HA/β-TCP groups was significantly greater than that of the empty control group (p<0.05), but without statistical difference from that of MBCP group. The degree of uniformity of new bone formation within defect region for PC-HA/β-TCP (60/40) was higher than that for MBCP. The PC-HA/β-TCP grafts showed enhanced bone regenerations with a more even dispersion of new bone formation than the other materials without causing inflammation, suggesting that these materials may be an alternative choice for bone void fillers in dental applications.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Process / drug effects*
  • Alveolar Process / surgery
  • Alveolar Process / ultrastructure
  • Analysis of Variance
  • Animals
  • Biocompatible Materials / administration & dosage*
  • Biocompatible Materials / chemistry
  • Bone Regeneration / drug effects
  • Bone Substitutes / administration & dosage*
  • Bone Substitutes / chemistry
  • Calcium Phosphates / administration & dosage*
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / therapeutic use
  • Crystallization
  • Dogs
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Hydroxyapatites / administration & dosage
  • Hydroxyapatites / chemistry
  • Osseointegration / drug effects*
  • Tooth Socket / drug effects
  • Tooth Socket / ultrastructure

Substances

  • Biocompatible Materials
  • Bone Substitutes
  • Calcium Phosphates
  • Drug Combinations
  • Hydroxyapatites