The subcellular distributions of amine oxidase activities MAO-A, MAO-B and SSAO, in bovine lung have been determined. MAO activities are largely confined to the mitochondria whereas SSAO activity is concentrated in the microsomal fraction. The contribution of different type of amine oxidase present in the microsomal fraction to the metabolism of the benzylamine, tyramine, phenylethylamine, tryptamine and dopamine were determined. The kinetic constants towards benzylamine showed SSAO to have a higher affinity than MAO-B, and the comparison of the Vmax/Km relationship for both enzymes indicated that benzylamine would be oxidised more efficiently by SSAO than by MAO-B.