New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry

J Proteomics. 2011 May 1;74(5):595-606. doi: 10.1016/j.jprot.2011.01.014. Epub 2011 Feb 2.

Abstract

This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI-TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC-MS/MS using an Agilent 6520 Accurate-Mass Q-TOF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / analysis*
  • Female
  • Hot Temperature
  • Humans
  • Male
  • Middle Aged
  • Peptides / analysis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Blood Proteins
  • Peptides