Aim: To analyze the effect of recombinant IL-1β on proliferation, migration, and the effect on IFNα induced cell growth inhibtion.
Methods: The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA. Cell migration was assessed using wound healing assay.
Results: IL-1β significantly stimulated proliferation and migration of Hepa1-6 cells. However, expression of IL-1β significantly down-regulated growth inhibition inducecd by IFNα.
Conclusion: The recombinant vector could stably express IL-1β and promote in vitro proliferation, migration, and impair IFNα-induced cell growth inhibition.