Three strains, K08-0182(T), K08-0178 and K08-0195 were isolated from the paste of ground plant roots collected in Kanagawa Prefecture, Japan. These strains contained meso-diaminopimelic acid and lysine as the diamino acids in cell-wall peptidoglycan, and MK-9(H(6)) and MK-9(H(8)) as the predominant menaquinones. The G+C contents of the DNA were 72-73 mol%. Taken together, these characteristics combined with 16S rRNA gene sequence analyses revealed that the isolated strains belong to the genus Actinoallomurus. DNA-DNA hybridization values showed that the three strains belonged to a novel species of the genus Actinoallomurus. Therefore strains K08-0182(T), K08-0178 and K08-0195 are proposed as representatives of a novel species, Actinoallomurus radicium sp. nov. The type strain is K08-0182(T) ( = DSM 45523(T) = NBRC 107678(T) = JCM 17294(T)).