Relative performance of common biochemical indicators in detecting cigarette smoking

Addiction. 2011 Jul;106(7):1325-34. doi: 10.1111/j.1360-0443.2011.03441.x. Epub 2011 May 27.

Abstract

Aims: Many cities have banned indoor smoking in public places. Thus, an updated recommendation for a breath carbon monoxide (CO) cut-off is needed that optimally determines smoking status. We evaluated and compared the performance of breath CO and semiquantitative cotinine immunoassay test strips (urine and saliva NicAlert®) alone and in combination.

Design: Cross-sectional study.

Setting: Urban drug addiction research and treatment facility.

Participants: Ninety non-treatment-seeking smokers and 82 non-smokers.

Measurements: Participants completed smoking histories and provided breath CO, urine and saliva specimens. Urine and saliva specimens were assayed for cotinine by NicAlert® and liquid chromatography-tandem mass spectrometry (LCMSMS).

Findings: An optimal breath CO cut-off was established using self-report and LCMSMS analysis of cotinine, an objective indicator, as reference measures. Performance of smoking indicators and combinations were compared to the reference measures. Breath CO ≥5 parts per million (p.p.m.) optimally discriminated smokers from non-smokers. Saliva NicAlert® performance was less effective than the other indicators.

Conclusions: In surveys of smokers and non-smokers in areas with strong smoke-free laws, the breath carbon monoxide cut-off that discriminates most effectively appears to be ≥5 p.p.m. rather than the ≥10 p.p.m. cut-off often used. These findings may not generalize to clinical trials, regions with different carbon monoxide pollution levels or areas with less stringent smoke-free laws.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Adolescent
  • Adult
  • Biomarkers / metabolism
  • Breath Tests
  • Carbon Monoxide / analysis*
  • Cotinine / analysis*
  • Cotinine / pharmacokinetics
  • Cross-Sectional Studies
  • Data Interpretation, Statistical
  • Female
  • Half-Life
  • Humans
  • Immunoassay / standards
  • Male
  • Reagent Kits, Diagnostic / standards*
  • Reference Standards
  • Saliva / chemistry*
  • Self Report
  • Sensitivity and Specificity
  • Smoking / legislation & jurisprudence
  • Smoking / metabolism*
  • Tandem Mass Spectrometry
  • Tobacco Smoke Pollution / analysis*
  • Tobacco Smoke Pollution / legislation & jurisprudence
  • Urban Population

Substances

  • Biomarkers
  • Reagent Kits, Diagnostic
  • Tobacco Smoke Pollution
  • Carbon Monoxide
  • Cotinine