The presence of researchers, ecotourists or rangers inside protected areas is generally assumed to provide a protective effect for wildlife populations, mainly by reducing poaching pressure. However, this assumption has rarely been empirically tested. Here, we evaluate and quantify the conservation benefits of the presence of a long-term research area in Taï National Park, Côte d'Ivoire. A wildlife survey following 225 km of line transects revealed considerably higher primate and duiker encounter rates within the research area when compared with adjacent areas. This positive effect was particularly pronounced for threatened and over-harvested species, such as the endangered red colobus monkey (Procolobus badius). This pattern was clearly mirrored by a reversed gradient in signs of poaching, which decreased towards and inside the research area, a trend that was also supported with park-wide data. This study demonstrates that even relatively simple evidence-based analytical approaches can bridge the gap between conservation theory and practice. In addition, it emphasizes the value of establishing long-term research sites as an integral part of protected area management.