Clinical, histopathologic, and cytologic diagnosis of mucosal leishmaniasis and literature review

Arch Pathol Lab Med. 2011 Apr;135(4):478-82. doi: 10.5858/2010-0069-OA.1.

Abstract

Context: Mucosal leishmaniasis (ML) is a rare disease in the world, even in endemic areas such as Iran. Clinical, histologic, or cytologic assessment may help in the diagnosis of ML.

Objective: To describe clinical, histologic, and cytologic findings in ML.

Design: Review of our files showed 11 patients diagnosed with ML, of whom 7 patients had oral lesions, 1 of whom was a known patient with oral leishmaniasis with recurrence of oral lesions; 2 had laryngeal lesions; and 3 had nasal lesions. One case of laryngeal leishmaniasis was a recurrence of prior oral lesions. Cytologic smears were prepared by scraping the lesions with a scalpel or cytobrush. Histology on the biopsies was done for 7 patients. In 2 patients with nasal lesions, exfoliative cytology was made by washing the nasal cavity. Smears were both air dried and fixed in alcohol and stained.

Results: Cytologic findings showed free Leishman-Donovan bodies, intrahistiocytic Leishman-Donovan bodies, atypical organisms, granuloma, acute and chronic inflammatory cells, histiocytes, multinucleated giant cells, mast cells, binucleated histiocytes (Reed-Sternberg-like cells), and plasma cells. In 6 of the patients, biopsy was inconclusive and in subsequent cytology the organism was detected. In 3 cases, findings from clinical and cytologic examinations were suggestive for leishmaniasis; however, with response to treatment, the diagnosis was confirmed. In 5 patients a malignant tumor was suspected because of clinical or histologic findings, but cytology helped to diagnose leishmaniasis.

Conclusions: Clinically or histologically, ML can be mistaken for benign and malignant lesions. Scraping or exfoliative cytology is an easy, reliable, and cost-effective method for diagnosing ML. Thus, clinical, histologic, and cytologic features together may help in ML diagnosis.

Publication types

  • Review

MeSH terms

  • Adult
  • Biopsy
  • Cytodiagnosis / methods*
  • Diagnosis, Differential
  • Female
  • Histiocytes / parasitology
  • Histiocytes / pathology
  • Humans
  • Leishmania / isolation & purification
  • Leishmaniasis, Mucocutaneous / diagnosis*
  • Leishmaniasis, Mucocutaneous / parasitology
  • Male
  • Middle Aged
  • Mouth Diseases / parasitology
  • Mouth Diseases / pathology
  • Mouth Neoplasms / diagnosis
  • Recurrence
  • Retrospective Studies
  • Young Adult