Development of a highly selective fluorescence probe for alkaline phosphatase

Mitsuyasu Kawaguchi; Kenjiro Hanaoka; Toru Komatsu; Takuya Terai; Tetsuo Nagano

doi:10.1016/j.bmcl.2011.03.070

Development of a highly selective fluorescence probe for alkaline phosphatase

Bioorg Med Chem Lett. 2011 Sep 1;21(17):5088-91. doi: 10.1016/j.bmcl.2011.03.070. Epub 2011 Mar 23.

Authors

Mitsuyasu Kawaguchi¹, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano

Affiliation

¹ Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

PMID: 21482108
DOI: 10.1016/j.bmcl.2011.03.070

Abstract

We have developed the first highly selective fluorescence probe for alkaline phosphatase (ALP), TG-mPhos. This probe shows selectivity for ALP over protein tyrosine phosphatase and protein serine/threonine phosphatase. Our previously developed TG-Phos, which has a phenolic phosphate linkage in place of the alcoholic phosphate linkage of TG-mPhos, lacks this selectivity. TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / chemistry*
Fluorescent Dyes / chemistry*
Photochemistry
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Alkaline Phosphatase