Enhanced translocation of poly(dt)45 through an α-hemolysin nanopore by binding with antibody

Chem Commun (Camb). 2011 May 28;47(20):5690-2. doi: 10.1039/c0cc05787h. Epub 2011 Apr 14.

Abstract

The translocation time of poly(dT)(45) through an α-hemolysin pore was reduced in the presence of a DNA-binding Fab fragment. The Fab acts as a rudder to steer the DNA into the pore.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • DNA / chemistry
  • Electrodes
  • Hemolysin Proteins / chemistry*
  • Hemolysin Proteins / metabolism
  • Immunoglobulin Fab Fragments / chemistry*
  • Immunoglobulin Fab Fragments / immunology
  • Nanopores*
  • Poly T / chemistry*
  • Poly T / immunology
  • Poly T / metabolism
  • Protein Binding

Substances

  • Hemolysin Proteins
  • Immunoglobulin Fab Fragments
  • Poly T
  • DNA