Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Hidemasa Izumiya; Kazutoshi Matsumoto; Shunsuke Yahiro; Jiyoung Lee; Masatomo Morita; Shouji Yamamoto; Eiji Arakawa; Makoto Ohnishi

doi:10.1016/j.mcp.2011.04.004

Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Mol Cell Probes. 2011 Aug;25(4):174-6. doi: 10.1016/j.mcp.2011.04.004. Epub 2011 Apr 22.

Authors

Hidemasa Izumiya¹, Kazutoshi Matsumoto, Shunsuke Yahiro, Jiyoung Lee, Masatomo Morita, Shouji Yamamoto, Eiji Arakawa, Makoto Ohnishi

Affiliation

¹ Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan. izumiya@nih.go.jp

PMID: 21530641
DOI: 10.1016/j.mcp.2011.04.004

Abstract

A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.

MeSH terms

Bacterial Typing Techniques / methods
Phylogeny
Polymerase Chain Reaction / methods*
Vibrio / classification*
Vibrio / genetics
Vibrio / isolation & purification*
Vibrio cholerae / classification
Vibrio cholerae / genetics
Vibrio cholerae / isolation & purification
Vibrio parahaemolyticus / classification
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / isolation & purification
Vibrio vulnificus / classification
Vibrio vulnificus / genetics
Vibrio vulnificus / isolation & purification