Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

Chem Commun (Camb). 2011 Jun 14;47(22):6257-9. doi: 10.1039/c1cc10743g. Epub 2011 May 6.

Abstract

Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry*
  • Azides / chemistry*
  • Click Chemistry
  • Copper / chemistry
  • Cyclization
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry
  • Oligonucleotides / chemistry

Substances

  • Alkynes
  • Azides
  • Fluorescent Dyes
  • Oligonucleotides
  • Copper
  • DNA