Abstract
The development and validation of methods for determining concentrations of the antipsychotic drug asenapine (ASE) and three of its metabolites [N-desmethylasenapine (DMA), asenapine-N(+) -glucuronide (ASG) and 11-O-sulfate-asenapine (OSA)] in human plasma using LC-MS/MS with automated solid-phase extraction is described. The three assessment methods in human plasma were found to be acceptable for quantification in the ranges 0.0250-20.0 ng/mL (ASE), 0.0500-20.0 ng/mL (DMA and OSA) and 0.250-50.0 ng/mL (ASG).
Copyright © 2011 John Wiley & Sons, Ltd.
Publication types
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Clinical Trial, Phase I
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Randomized Controlled Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Chromatography, Liquid / methods*
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Dibenzocycloheptenes
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Double-Blind Method
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Drug Stability
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Heterocyclic Compounds, 4 or More Rings / blood*
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Heterocyclic Compounds, 4 or More Rings / metabolism
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Heterocyclic Compounds, 4 or More Rings / pharmacokinetics
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Humans
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Linear Models
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Male
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Middle Aged
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Reference Standards
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Reproducibility of Results
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Sensitivity and Specificity
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Solid Phase Extraction / methods*
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Tandem Mass Spectrometry / methods*
Substances
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Dibenzocycloheptenes
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Heterocyclic Compounds, 4 or More Rings
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asenapine