Characterization of microsatellite loci in the fungus, Grosmannia clavigera, a pine pathogen associated with the mountain pine beetle

C K M Tsui; N Feau; C E Ritland; S Massoumi Alamouti; S Diguistini; L Khadempour; J Bohlmann; C Breuil; R C Hamelin

doi:10.1111/j.1755-0998.2009.02717.x

Characterization of microsatellite loci in the fungus, Grosmannia clavigera, a pine pathogen associated with the mountain pine beetle

Mol Ecol Resour. 2009 Nov;9(6):1500-3. doi: 10.1111/j.1755-0998.2009.02717.x. Epub 2009 May 5.

Authors

C K M Tsui¹, N Feau, C E Ritland, S Massoumi Alamouti, S Diguistini, L Khadempour, J Bohlmann, C Breuil, R C Hamelin

Affiliation

¹ Department of Forest Science, The University of British Columbia, Vancouver, BC, Canada V6T 1Z4 Natural Resources Canada, 1055 rue du P.E.P.S., Ste-Foy, QC, Canada G1V 4C7 Department of Wood Science, The University of British Columbia, Vancouver, BC, Canada V6T 1Z4 Michael Smith Laboratories, The University of British Columbia, Vancouver, BC, Canada V6T 1Z4.

PMID: 21564944
DOI: 10.1111/j.1755-0998.2009.02717.x

Abstract

The largest forest pest epidemic in Canadian history caused by the mountain pine beetle (MPB) and its fungal associates has killed over 15 million hectares of forest. Sixty simple sequence repeat regions were identified from Grosmannia clavigera, an MPB associated fungus. Eight loci genotyped in 53 isolates from two populations in British Columbia, Canada revealed three to 10 alleles per locus and gene diversities of 0 to 0.79. All but two of these loci showed length polymorphism in Leptographium longiclavatum, a related MPB fungal associate. These microsatellites will be useful in population genetic studies of these fungi.