Microsatellite primers for the four Galápagos mockingbird species (Mimus parvulus, Mimus macdonaldi, Mimus melanotis and Mimus trifasciatus)

P E A Hoeck; T B Bucher; P Wandeler; L F Keller

doi:10.1111/j.1755-0998.2009.02704.x

Microsatellite primers for the four Galápagos mockingbird species (Mimus parvulus, Mimus macdonaldi, Mimus melanotis and Mimus trifasciatus)

Mol Ecol Resour. 2009 Nov;9(6):1538-41. doi: 10.1111/j.1755-0998.2009.02704.x. Epub 2009 Apr 15.

Authors

P E A Hoeck¹, T B Bucher, P Wandeler, L F Keller

Affiliation

¹ Zoological Museum, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.

PMID: 21564952
DOI: 10.1111/j.1755-0998.2009.02704.x

Abstract

Nineteen di- and tetranucleotide and one trinucleotide microsatellite DNA markers were isolated from the Galápagos mockingbird (Mimus parvulus) and tested for cross-species amplification in the other three mockingbird species in the Galápagos. In addition, primers for two microsatellite loci previously developed for Mimus polyglottos were redesigned to obtain shorter amplification fragments. The number of alleles per locus and species ranged from 1 to 8, and expected heterozygosity varied from 0.0 to 0.809. These microsatellite markers will be useful to study levels of inbreeding in different island populations.