Combined application of PCR-based functional assays for the detection of aromatic-compound-degrading anaerobes

Appl Environ Microbiol. 2011 Jul;77(14):5056-61. doi: 10.1128/AEM.00335-11. Epub 2011 May 20.

Abstract

To explore the reliability of assays that detect aromatic-compound-degrading anaerobes, a combination of three functional-gene-targeting assays was applied to microcosms from benzene-contaminated aquifers. Results of the assays were consistent and suggest that species related to the genera Azoarcus and Geobacter dominated benzene degradation at the individual sites.

MeSH terms

  • Acyl Coenzyme A / metabolism
  • Azoarcus / genetics*
  • Azoarcus / metabolism
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification
  • Bacteria, Anaerobic / metabolism
  • Benzene / metabolism
  • Biodegradation, Environmental
  • Carbon-Carbon Lyases / genetics
  • DNA, Bacterial / genetics
  • Geobacter / genetics*
  • Geobacter / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics
  • Water Pollutants, Chemical / metabolism*
  • Water Pollution

Substances

  • Acyl Coenzyme A
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Water Pollutants, Chemical
  • benzoyl-coenzyme A
  • Carbon-Carbon Lyases
  • benzylsuccinate synthase
  • Benzene

Associated data

  • GENBANK/GU057387
  • GENBANK/GU057388
  • GENBANK/GU057389
  • GENBANK/GU057390
  • GENBANK/GU057391
  • GENBANK/GU057392
  • GENBANK/GU057393
  • GENBANK/GU057394
  • GENBANK/GU057395
  • GENBANK/GU057396
  • GENBANK/GU057397
  • GENBANK/GU057398
  • GENBANK/GU057399
  • GENBANK/GU057400
  • GENBANK/GU057401
  • GENBANK/GU057402
  • GENBANK/GU057403
  • GENBANK/GU057404
  • GENBANK/GU057405
  • GENBANK/HQ640662
  • GENBANK/HQ640663
  • GENBANK/HQ640664
  • GENBANK/HQ640665
  • GENBANK/HQ640666
  • GENBANK/HQ640667
  • GENBANK/HQ640668
  • GENBANK/HQ640669
  • GENBANK/HQ640670
  • GENBANK/HQ640671
  • GENBANK/HQ640672
  • GENBANK/HQ640673
  • GENBANK/HQ640674
  • GENBANK/HQ640675
  • GENBANK/HQ640676