Abstract
Nerve growth factor (NGF) affects levels of the alpha subunit of the stimulatory G protein (Gs-alpha) in pheochromocytoma 12 cells in a bidirectional, density-dependent manner. Cells grown at high density responded to NGF treatment with increased levels of Gs-alpha mRNA and protein. Conversely, in cells grown in low-density cultures, levels of this mRNA were lowered by NGF treatment.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adrenal Gland Neoplasms
-
Animals
-
Cell Division / drug effects
-
Cell Line
-
Cholera Toxin / pharmacology
-
Cyclic AMP / metabolism
-
GTP-Binding Proteins / genetics*
-
Gene Expression Regulation, Neoplastic / drug effects*
-
Macromolecular Substances
-
Nerve Growth Factors / pharmacology*
-
Pheochromocytoma
-
RNA, Messenger / drug effects
-
RNA, Messenger / genetics*
-
RNA, Neoplasm / drug effects
-
RNA, Neoplasm / genetics
-
Rats
-
Transcription, Genetic / drug effects
-
Tumor Cells, Cultured / cytology
-
Tumor Cells, Cultured / drug effects*
-
Tumor Cells, Cultured / metabolism
Substances
-
Macromolecular Substances
-
Nerve Growth Factors
-
RNA, Messenger
-
RNA, Neoplasm
-
Cholera Toxin
-
Cyclic AMP
-
GTP-Binding Proteins