Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

Am J Bot. 2011 Apr;98(4):e81-3. doi: 10.3732/ajb.1000356. Epub 2011 Mar 30.

Abstract

Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.

Methods and results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.

Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Australia
  • Cloning, Molecular
  • DNA Primers*
  • DNA, Plant / analysis*
  • Diet
  • Ecosystem
  • Genetic Loci*
  • Microsatellite Repeats*
  • Polymorphism, Genetic*
  • Polyploidy*
  • Solanum / genetics*
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Plant