A phase I study of the safety and pharmacokinetics of the hypoxia-activated prodrug TH-302 in combination with doxorubicin in patients with advanced soft tissue sarcoma

Oncology. 2011;80(1-2):50-6. doi: 10.1159/000327739. Epub 2011 May 31.

Abstract

Purpose: The purpose of this study was to determine the dose-limiting toxicities (DLT), maximum tolerated dose (MTD), safety, pharmacokinetics and preliminary activity of TH-302, a hypoxia-activated prodrug, in combination with doxorubicin in patients with advanced soft tissue sarcoma.

Patients and methods: TH-302 was administered intravenously on days 1 and 8 and doxorubicin 75 mg/m² on day 1 (2 h after TH-302) of every 3-week cycle. TH-302 starting dose was 240 mg/m² with a classic 3 + 3 dose escalation. Pharmacokinetics were assessed on days 1 and 8 of cycle 1. Tumor assessments were performed after every second cycle.

Results: Sixteen patients enrolled. Prophylactic growth factor support was added due to grade 4 neutropenia. The MTD was 300 mg/m². DLTs at 340 mg/m² were neutropenia-associated infection and grade 4 thrombocytopenia. Common adverse events included fatigue, nausea and skin rash. There was no evidence of pharmacokinetic interaction between TH-302 and doxorubicin. Five of 15 (33%) evaluable patients had a partial response by RECIST (Response Evaluation Criteria in Solid Tumors) criteria.

Conclusions: The hematologic toxicity of doxorubicin is increased when combined with TH-302. This can be mitigated by prophylactic growth factor support. Toxicities were manageable and there was evidence of antitumor activity.

Publication types

  • Clinical Trial, Phase I
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscess / etiology*
  • Adult
  • Aged
  • Aged, 80 and over
  • Anemia / chemically induced
  • Antineoplastic Combined Chemotherapy Protocols / adverse effects*
  • Antineoplastic Combined Chemotherapy Protocols / pharmacokinetics
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Cellulitis / chemically induced
  • Disease-Free Survival
  • Doxorubicin / adverse effects*
  • Doxorubicin / analogs & derivatives
  • Doxorubicin / metabolism
  • Doxorubicin / pharmacokinetics
  • Doxorubicin / therapeutic use
  • Drug Eruptions
  • Female
  • Granulocyte Colony-Stimulating Factor / therapeutic use
  • Humans
  • Lymphopenia / chemically induced
  • Male
  • Maximum Tolerated Dose*
  • Middle Aged
  • Neoadjuvant Therapy
  • Neutropenia / chemically induced*
  • Neutropenia / drug therapy
  • Nitroimidazoles / adverse effects*
  • Nitroimidazoles / pharmacokinetics
  • Nitroimidazoles / therapeutic use
  • Phosphoramide Mustards / adverse effects*
  • Phosphoramide Mustards / pharmacokinetics
  • Phosphoramide Mustards / therapeutic use
  • Sarcoma / drug therapy*
  • Sarcoma / surgery
  • Stomatitis / chemically induced
  • Treatment Outcome
  • Young Adult

Substances

  • Nitroimidazoles
  • Phosphoramide Mustards
  • TH 302
  • Granulocyte Colony-Stimulating Factor
  • Doxorubicin
  • adriamycinol