Protective effect of magnesium-25 carrying porphyrin-fullerene nanoparticles on degeneration of dorsal root ganglion neurons and motor function in experimental diabetic neuropathy

Basic Clin Pharmacol Toxicol. 2011 Nov;109(5):381-6. doi: 10.1111/j.1742-7843.2011.00741.x. Epub 2011 Jul 12.

Abstract

Diabetic neuropathy (DN) is the most common peripheral neuropathy and long-term complication of diabetes. In view of the pathological basis for the treatment of DN, it is important to prevent nerve degeneration. Most of the current treatment strategies are symptomatic therapies. In this study, we evaluated the effectiveness of magnesium-25, carrying porphyrin-fullerene nanoparticles, on diabetes-induced neuropathy. Previous studies have suggested that dorsal root ganglion (DRG) neurons comprise a specific target and may be responsible for the known complications of DN. Experimental DN was induced by intraperitoneal injection of streptozotocin (STZ) (45 mg/kg). Different forms of magnesium including (25)Mg-PMC16, (24)Mg-PMC16 and MgCl(2) were administered intravenously in equal dose (0.5 LD(50)) at 48-hr intervals before STZ injection. Peripheral nerves were studied after 2 months of diabetes in groups using qualitative approaches, morphometric analysis of DRG neurons and motor function tests. We showed that STZ-induced DN caused morphological abnormalities in DRG neurons comprising changes in area, diameter and number of A and B cells as well as motor dysfunction in DN. Moreover, our findings indicated that administration of (25)Mg-PMC16 as a magnetic form of Mg improved morphological abnormalities and motor dysfunctions significantly, whereas other forms of Mg were ineffective.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetes Mellitus, Experimental / pathology
  • Diabetic Neuropathies / drug therapy*
  • Diabetic Neuropathies / pathology*
  • Dose-Response Relationship, Drug
  • Drug Delivery Systems
  • Fullerenes / administration & dosage*
  • Ganglia, Spinal / drug effects*
  • Magnesium / administration & dosage*
  • Male
  • Metal Nanoparticles / chemistry
  • Neurons / pathology
  • Oxidative Stress / drug effects
  • Peripheral Nerves / drug effects
  • Peripheral Nerves / pathology
  • Porphyrins / administration & dosage*
  • Rats
  • Streptozocin / metabolism

Substances

  • Biomarkers
  • Fullerenes
  • Porphyrins
  • Streptozocin
  • Magnesium