[Molecular and clinical characterization of hepatitis a virus in Gwangju and Jeonnam province]

Korean J Gastroenterol. 2011 Jun;57(6):346-51. doi: 10.4166/kjg.2011.57.6.346.
[Article in Korean]

Abstract

Background/aims: Dominant genotype of hepatitis A virus (HAV) in Korea had been known to be genotype 1A in 1990s. Recently, the epidemiologic change of HAV genotype was reported with an upsurge of acute hepatitis A in Korea. The aim of this study was to investigate the change of HAV genotypes and clinical characteristics in Gwangju and Jeonnam province.

Methods: From November 2008 to October 2009, a total of 82 patients with acute hepatitis A were enrolled prospectively. HAV genotype was determined using reverse transcriptase polymerase chain reaction and sequencing of the PCR products of VP1/2A region of HAV.

Results: HAV RNA was detected in 82% (67/82). The genotype IIIA (88%, 59 cases) was significantly more frequent than genotype IA (12%, 8 cases) (p ≤ 0.01). The subtypes of genotype IIIA were AJ299467 Norway (49%), HA-JNG04-09 Japan (27%), HS-14-12-00 Spain (22%) and H-122 Sweden (2%). The subtypes of IA were FH1 Japan (50%) and HA J04-3 Japan (50%). The substitutions of amino acid were more frequent in genotype IIIA than IA (p≤ 0.01). There was no difference in the clinical characteristics between the patients with genotype IIIA and IA.

Conclusions: Genotype IIIA was a dominant genotype of recent HAV infection in Gwangju and Jeonnam province. This study provides valuable epidemiologic information of genetic distributions of HAV in Korea.

Publication types

  • English Abstract

MeSH terms

  • Acute Disease
  • Adult
  • Female
  • Genotype
  • Hepatitis A / diagnosis
  • Hepatitis A / virology*
  • Hepatitis A virus / classification
  • Hepatitis A virus / genetics*
  • Humans
  • Korea
  • Male
  • Phylogeny
  • Prospective Studies
  • RNA, Viral / analysis
  • Rural Population
  • Sequence Analysis, RNA
  • Urban Population
  • Viral Structural Proteins / genetics

Substances

  • RNA, Viral
  • VP1 protein, hepatitis A virus
  • Viral Structural Proteins