Enteroviruses were isolated from 12% of the digestive tract samples collected from Anodonta cygnea (9.4%) and Dreysena polymorpha (13.2%) shell species and from 8.4% of the branchiae sample from fish species Rutilus rutilus, Perca fluviatilis and Silurus glanis. The isolated belonged to the following antigenic types: ECHO 2 and 7, Coxsackie B 2, 4 and 6, and poliovirus 1 and 2.