Abstract
Northern analysis was employed to investigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif structural genes and in the intergenic noncoding regions. The results indicate that intergenic RNA secondary structures effect the differential accumulation of transcripts, supporting the high Fe protein-to-MoFe protein ratio required for optimal diazotrophic growth.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Azotobacter vinelandii / genetics*
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Azotobacter vinelandii / growth & development
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Molybdoferredoxin / chemistry
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Molybdoferredoxin / genetics
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Molybdoferredoxin / metabolism
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Multigene Family
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Mutation
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Nitrogen Fixation
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Nucleic Acid Conformation
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RNA, Bacterial / genetics
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
Substances
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Bacterial Proteins
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Molybdoferredoxin
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RNA, Bacterial
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RNA, Messenger